| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | venezuelae with a ReDirect disrupted sven6563::apr rsrR::apr 3xFlag RsrR rsrR::apr with a pMS82 encoded N-terminal 3xFlag tagged rsrR with 300 bp of upstream flanking DNA (promoter) Plasmids pIJ773 SV-5-F05 pMS82 pGS-21a pJM026 pBluescript KS (+ ), aac(3)IV, oriT (RK2), FRT sites Supercos-1-cosmid with (a 52181 bp) fragment containing sven6562/3 ori, pUC18, hyg, oriT, RK2, int Φ BT1 Genscript overexpression and purification vector (SD0121) SV-5-F05 containing sven6563::apr oriT pMS82, rsrR gene plus 300 bp upstream DNA with a c-terminal synthetic linker and 3xFLAG tag pGS-21a, full length rsrR cloned NdeI/XhoI pJM028 with a c-terminal 6xHis tag NdeI/XhoI pJM028 with a c-terminal synthetic linker as with (flag), 2xFLAG tag and a 6xHis tag, cloned NdeI/XhoI pJM027 pJM028 pJM029 pJM030 Source Invitrogen 48,58 59,60 61 4 This work 48 4 62 Genscript This work This work This work This work This work Table 2. | Get A Quote |
Members of the Rrf2 superfamily of transcription factors are widespread in bacteria but their functions are largely unexplored. The few that have been characterized in detail sense nitric oxide (NsrR), iron limitation (RirA), cysteine availability (CymR) and the iron sulfur (Fe-S) cluster status of the cell (IscR). In this study we combined ChIP- and dRNA-seq with in vitro biochemistry to characterize a putative NsrR homologue in Streptomyces venezuelae. ChIP-seq analysis revealed that rather than regulating the nitrosative stress response like Streptomyces coelicolor NsrR, Sven6563 binds to a conserved motif at a different, much larger set of genes with a diverse range of functions, including a number of regul... More
